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Strain Name
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C57BL/6N-Gdf15tm1(GDF15)Bcgen/Bcgen
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Common Name
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B-hGDF15 mice
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Background
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C57BL/6N
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Catalog number
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111201
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Related Genes
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growth differentiation factor 15, GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
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NCBI Gene ID
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23886
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mRNA expression analysis
Strain specific analysis of GDF15 gene expression in wild-type mice and B-hGDF15 mice by RT-PCR. Mouse Gdf15 mRNA was detectable in kidney of wild-type (+/+). Human GDF15 mRNA was detectable only in H/H but not in +/+ mice.
Protein expression analysis
Strain-specific GDF15 expression analysis in wild-type C57BL/6 mice and homozygous B-hGDF15 mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF15 mice (H/H), and analyzed by ELISA with species-specific GDF15 ELISA kit (R&D, Mouse/Rat GDF-15 Quantikine ELISA Kit: MGD150, Human GDF-15 Quantikine ELISA Kit: DGD150). Mouse GDF15 was exclusively detectable in wild-type mice and human GDF15 was exclusively detectable in homozygous B-hGDF15 mice. Values are expressed as mean ± SEM.
Tumor growth curve & body weight changes
Subcutaneous tumor growth of B-hGDF15 MC38 cells. B-hGDF15 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into homozygous B-hGDF15 mice (female, 6-8 weeks old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. The B-hGDF15 MC38 cell inoculation group shows a decreasing trend in body weight compared to the wild-type MC38 cell inoculation group. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. Results indicate that B-hGDF15 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
Note: The B-hGDF15 MC38 tumor was only implanted in B-hGDF15 mice and has not been implanted in C57BL/6 mice.
In vivo efficacy of anti-human GDF15 antibodies
Antitumor activity of anti-human GDF15 antibody (ponsegromab analog, in-house) in B-hGDF15 mice. (A) B-hGDF15 MC38 tumor growth in B-hGDF15 mice. Murine colon cancer B-hGDF15 MC38 cells (5×105) were subcutaneously implanted into homozygous B-hGDF15 mice (female, 9 weeks old, n=6). Mice were grouped when tumor volume reached approximately 100-150 mm3, at which time they were intraperitoneally injected with anti-human GDF15 antibody indicated in panel. (B) Body weight changes during treatment. (C) The weight change of each mouse in the group compared to Day 0. As shown in panel B and C, anti-human GDF15 antibody was effective in controlling the weight loss induced by B-hGDF15 MC38 in B-hGDF15 mice, demonstrating that the B-hGDF15 mice and B-hGDF15 MC38 provide a powerful preclinical model for in vivo evaluation of anti-human GDF15 antibodies. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001.
The overage of this tumor model is 50%.
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