B-hB7-H3 mice (C)
| Strain Name |
BALB/cCrSlcNifdc-Cd276tm2(CD276)Bcgen/Bcgen
|
Common Name | B-hB7-H3 mice (C) |
| Background | BALB/cCrSlcNifdc | Catalog number | 111806 |
| Aliases | CD276: 4Ig-B7-H3, B7-H3, B7H3, B7RP-2 | ||
Strain specific B7-H3 expression analysis in homozygous B-hB7-H3 mice (C) by western blot. Tissues were collected from wild-type BALB/c mice (+/+) and homozygous B-hB7-H3 mice (C) (H/H), and analyzed by western blot with anti-B7-H3 antibodies. Human B7-H3 was detectable in homozygous B-hB7-H3 mice (C) but not in wild-type BALB/c mice.
mRNA expression analysis
Strain specific analysis of B7-H3 mRNA expression in wild-type BALB/c mice and B-hB7-H3 mice (C) by RT-PCR. Spleen RNA was isolated from wild-type BALB/c mice (+/+) and homozygous B-hB7-H3 mice (C) (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human B7-H3 primers. Mouse B7h3 mRNA was detectable only in wild-type BALB/c mice. Human B7-H3 mRNA was detectable only in homozygous B-hB7-H3 mice (C) but not in wild-type mice.
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