Strain Name
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C57BL/6JNifdc-Cd164tm1(CD164)Bcgen/Bcgen
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Common Name
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B-hCD164 mice
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Background
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C57BL/6JNifdc
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Catalog Number
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111483
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Aliases
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DFNA66, MGC-24, MGC-24v, MUC-24, endolyn
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NCBI Gene ID
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8763
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Protein expression analysis in spleen
Strain specific CD164 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD164 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD164 mice (H/H). Protein expression was analyzed with anti-human CD164 antibody (Biolegend, 324808) by flow cytometry. Human CD164 was exclusively detectable in homozygous B-hCD164 mice, but not in wild-type C57BL/6 mice.
Strain specific CD164 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD164 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD164 mice (H/H). Protein expression was analyzed with anti-human CD164 antibody (Biolegend, 324808) by flow cytometry. Human CD164 was exclusively detectable in homozygous B-hCD164 mice, but not in wild-type C57BL/6 mice.
Protein expression analysis in BM
Strain specific CD164 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD164 mice by flow cytometry. BM were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD164 mice (H/H). Protein expression was analyzed with anti-human CD164 antibody (Biolegend, 324808) by flow cytometry. Human CD164 was exclusively detectable in homozygous B-hCD164 mice, but not in wild-type C57BL/6 mice.
Strain specific CD164 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD164 mice by flow cytometry. BM were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD164 mice (H/H). Protein expression was analyzed with anti-human CD164 antibody (Biolegend, 324808) by flow cytometry. Human CD164 was exclusively detectable in homozygous B-hCD164 mice, but not in wild-type C57BL/6 mice.
Protein expression analysis in blood
Strain specific CD164 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD164 mice by flow cytometry. Blood were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD164 mice (H/H). Protein expression was analyzed with anti-human CD164 antibody (Biolegend, 324808) by flow cytometry. Human CD164 was exclusively detectable in homozygous B-hCD164 mice, but not in wild-type C57BL/6 mice.
Strain specific CD164 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD164 mice by flow cytometry. Blood were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD164 mice (H/H). Protein expression was analyzed with anti-human CD164 antibody (Biolegend, 324808) by flow cytometry. Human CD164 was exclusively detectable in homozygous B-hCD164 mice, but not in wild-type C57BL/6 mice.
mRNA expression analysis
Strain specific analysis of CD164 mRNA expression in wild-type C57BL/6JNifdc mice and B-hCD164 mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD164 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse and human Cd164 primers. Mouse Cd164 mRNA was only detectable in wild-type mice but not in homozygous B-hCD164 mice. Human CD164 mRNA was only detectable in homozygous B-hCD164 mice but not in wild-type mice.