B-hCD16A mice(C)
| Strain Name |
BALB/c-Fcgr4tm2(FCGR3A)Bcgen/Bcgen |
Common Name | B-hCD16A mice(C) |
| Background | BALB/cCrSlcNifdc | Catalog number | 111885 |
|
Related Genes |
FCGR3A (Fc fragment of IgG receptor IIIa), CD16A | ||
Protein expression analysis in blood and spleen-NK cells
Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Blood and spleen were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was not detectable in wild-type mice. Human CD16A was only detectable in homozygous B-hCD16A mice(C) blood(A) and spleen(B).
Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Blood and spleen were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was not detectable in wild-type mice. Human CD16A was only detectable in homozygous B-hCD16A mice(C) blood(A) and spleen(B).
Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Blood and spleen were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was detectable in wild-type mice. Human CD16A was weakly detectable in homozygous B-hCD16A mice(C) blood(A) and spleen(B).
Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Blood and spleen were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was detectable in wild-type mice. Human CD16A was only detectable in homozygous B-hCD16A mice(C) blood(A) and spleen(B).
Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Peritoneal exudative macrophages(PEMs) were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was detectable in wild-type mice. Human CD16A was only detectable in homozygous B-hCD16A mice(C).
Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female BALB/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.
Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.
Analysis of LNs leukocyte subpopulations by FACS. LNs were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the LNs was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.
Analysis of LNs T cell subpopulations by FACS. LNs were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the LNs was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.
Analysis of blood leukocyte subpopulations by FACS. Blood were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.
Analysis of blood T cell subpopulations by FACS. Blood were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.
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