B-hCD28/hB7-H3 mice
| Strain Name |
C57BL/6-Cd28tm1(CD28)Bcgen Cd276tm1(CD276)Bcgen/Bcgen
|
Common Name | B-hCD28/hB7-H3 mice |
| Background | C57BL/6 | Catalog number | 121251 |
|
Related Genes |
CD28, Tp44, CD28 molecule; CD276, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2, CD276 molecule |
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|
NCBI Gene ID |
12487,102657 |
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Targeting strategy
Gene targeting strategy for B-hCD28/hB7-H3 mice. The exons 2-3 of mouse Cd28 gene that encode the extracellular domain were replaced by human CD28 exons 2-3 in B-hCD28/hB7-H3 mice. The exons 3~4 of mouse Cd276 gene that encode the extracellular domain were replaced by human CD276 exons 3~6 in B-hCD28/hB7-H3 mice.
Protein expression analysis
Strain specific CD28 expression analysis in homozygous B-hCD28/hB7-H3 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD28/hB7-H3 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD28 antibody. Mouse CD28 was detectable in WT mice (+/+). Human CD28 was exclusively detectable in homozygous B-hCD28/hB7-H3 mice (H/H;H/H) but not in WT mice (+/+).
Strain specific B7-H3 expression analysis in homozygous B-hCD28/hB7-H3 mice by western blot. Epididymis were collected from wild type (WT) mice (+/+) and homozygous B-hCD28/hB7-H3 mice (H/H;H/H), and analyzed by western blot with anti-B7-H3 antibody. B7-H3 was detectable in WT mice (+/+) and homozygous B-hCD28/hB7-H3 mice (H/H;H/H) due to the cross-reactivity of antibodies.
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