Strain Name
|
C57BL/6JNifdc-Itgb3tm2(ITGB3)Bcgen/Bcgen
|
Common Name
|
B-hITGB3 mice
|
Background
|
C57BL/6JNifdc
|
Catalog number
|
112329
|
Aliases
|
GT; GT2; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; BDPLT24
|
Protein expression analysis
Strain specific hITGB3 expression analysis in homozygous B-hITGB3 mice by flow cytometry. Blood platelet was collected from wild-type mice (+/+) and homozygous B-hITGB3 mice (H/H) and analyzed by flow cytometry with species-specific anti-ITGB3 antibody. Mouse ITGB3 was detectable in wild-type mice. Human ITGB3 was detectable in homozygous B-hITGB3 mice.
Strain specific hITGB3 expression analysis in homozygous B-hITGB3 mice by flow cytometry. Bone marrow/spleen/blood were collected from wild-type mice (+/+) and homozygous B-hITGB3 mice (H/H) and analyzed by flow cytometry with species-specific anti-ITGB3 antibody. Mouse ITGB3 was detectable in wild-type mice. Human ITGB3 was detectable in homozygous B-hITGB3 mice.
Strain specific hITGB3 expression analysis in homozygous B-hITGB3 mice by flow cytometry. Spleen and blood were collected from wild-type mice (+/+) and homozygous B-hITGB3 mice (H/H) and analyzed by flow cytometry with species-specific anti-ITGB3 antibody. Mouse ITGB3 was detectable in spleen and blood of wild-type mice. Human ITGB3 was detectable in spleen and blood of homozygous B-hITGB3 mice.
Strain specific hITGB3 expression analysis in homozygous B-hITGB3 mice by flow cytometry. Spleen and blood were collected from wild-type mice (+/+) and homozygous B-hITGB3 mice (H/H) and analyzed by flow cytometry with species-specific anti-ITGB3 antibody. Mouse ITGB3 was detectable in spleen and blood of wild-type mice. Human ITGB3 was detectable in blood of homozygous B-hITGB3 mice.
Strain specific hITGB3 expression analysis in homozygous B-hITGB3 mice by flow cytometry. Spleen and blood were collected from wild-type mice (+/+) and homozygous B-hITGB3 mice (H/H) and analyzed by flow cytometry with species-specific anti-ITGB3 antibody. Mouse ITGB3 was detectable in spleen and blood of wild-type mice. Human ITGB3 was detectable in blood of homozygous B-hITGB3 mice.
Strain specific hITGB3 expression analysis in homozygous B-hITGB3 mice by flow cytometry. Spleen and blood were collected from wild-type mice (+/+) and homozygous B-hITGB3 mice (H/H) and analyzed by flow cytometry with species-specific anti-ITGB3 antibody. Mouse ITGB3 was detectable in spleen and blood of wild-type mice. Human ITGB3 was detectable in blood of homozygous B-hITGB3 mice.
mRNA expression analysis
Strain specific analysis of ITGB3 mRNA expression in wild-type C57BL/6 mice and B-hITGB3 mice by RT-PCR. Spleen RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hITGB3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ITGB3 primers. Mouse ITGB3 mRNA was detectable only in wild-type C57BL/6 mice. Human ITGB3 mRNA was detectable only in homozygous B-hITGB3 mice but not in wild-type mice.