Strain Name
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C57BL/6N-Robo4tm1(ROBO4)Bcgen/Bcgen
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Common Name
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B-hROBO4 mice
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Background
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C57BL/6N
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Catalog number
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111022
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Related Genes
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AOVD3, ECSM4, MRB(magic roundabout)
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NCBI Gene ID
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74144
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mRNA expression analysis
Strain specific analysis of ROBO4 gene expression in wild-type C57BL/6 mice mice and homozygous hROBO4 mice by RT-PCR. Mouse Robo4 mRNA was detectable in wild-type C57BL/6 mice (+/+). Human ROBO4 mRNA was detectable only in homozygous B-hROBO4 but not in wild-type mice.
Protein expression analysis
Strain specific ROBO4 expression analysis in homozygous B-hROBO4 mice by western blot. Lung tissues were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hROBO4 mice (H/H), and analyzed by western blot with anti-ROBO4 antibody. Human ROBO4 was exclusively detectable in homozygous B-hROBO4 mice.