Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hTNFR2 mice(C) (H/H) stimulated with anti-mCD3ɛ antibody (7.5μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific TNFR2 antibody. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C).

Strain specific TNFR2 expression analysis in wild-type and B-hTNFR2 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hTNFR2 mice(C) (H/H) stimulated with anti-mCD3ɛ antibody (7.5μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific TNFR2 antibody. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 mice(C).

Antitumor activity of anti-human TNFR2 antibody in B-hTNFR2 mice(C). (A) Anti-human TNFR2 antibody inhibited CT26.WT tumor growth in B-hTNFR2 mice(C). Murine colon cancer CT26.WT cells were subcutaneously implanted into homozygous B-hTNFR2 mice(C) (female, 7-week-old, n=6). Mice were grouped when tumor volume reached approximately 40-60 mm3, at which time they were intraperitoneally injected with anti-human TNFR2 antibody indicated in panel. (B) Body weight changes during treatment. As shown in panel A, anti-human TNFR2 antibody was efficacious in controlling tumor growth in B-hTNFR2 mice(C), demonstrating that the B-hTNFR2 mice(C) provide a powerful preclinical model for in vivo evaluation of anti-human TNFR2 antibodies. Values are expressed as mean ± SEM. (The hTNFR2 Ab1 was provided by the client)