| Strain Name |
C57BL/6N-F12tm1(F12)Bcgen/Bcgen
|
Common Name | B-hFXII mice |
| Background | C57BL/6N | Catalog number |
112750 |
| Aliases |
HAE3, HAEX, HAF |
||
|
NCBI Gene ID |
2161 | ||
Mechanism of action
Coagulation is triggered by either extrinsic or intrinsic pathways, leading to the generation of thrombin. The extrinsic pathway is initiated during the formation of the TF-FVIIa complex, which activates FX to FXa via FIXa. The intrinsic pathway is initiated by the activation of Factor XII (FXII) mediated by negatively charged surfaces and promotes the generation of thrombin through consecutive activations of FXI, FIX, FX and prothrombin.
Targeting strategy
The exons 1~14 of mouse FXII gene that encode full-length protein were replaced by the exons 1~14 of human FXII gene in B-hFXII mice.
- √: The experiment is planned.
- NA: The experiment is not currently planned.
- According to the characteristics and applications of the products, you can add experiments in the table by yourself.
mRNA expression analysis in humanized B-hFXII mice
Strain specific analysis of FXII mRNA expression in wild-type C57BL/6N mice and B-hFXII mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6N mice (+/+) and heterozygous B-hFXII mice (H/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FXII primers. Mouse FXII mRNA were detectable in wild-type C57BL/6N and heterozygous B-hFXII mice. Human FXII mRNA was only detectable in heterozygous B-hFXII mice.
Protein expression analysis in humanized B-hFXII mice
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