B-hHTR3A mice
| Strain Name |
C57BL/6JNifdc-Htr3atm1(HTR3A)Bcgen/Bcgen
|
Common Name | B-hHTR3A mice |
| Background | C57BL/6JNifdc | Catalog number |
112973 |
|
Related Genes |
HTR3A; HTR3, 5HT3R, 5-HT-3, 5-HT3A, 5-HT3R |
||
|
NCBI Gene ID |
3359 | ||
- HTR3A belongs to the ligand-gated ion channel receptor superfamily. It encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. HTR3A causes fast, depolarizing responses in neurons after activation. Alternatively spliced transcript variants encoding different isoforms have been identified.
- HTR3A gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.
Targeting strategy
Protein expression analysis
Western blot analysis of HTR3A protein expression in homozygous B-hHTR3A mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hHTR3A mice (H/H), and then analyzed by western blot with anti-HTR3A antibody(Abcam, ab13897). 40 μg total proteins were loaded for western blotting analysis. hHTR3A was detected in brain, stomach, small intestine and colon.
Strain specific analysis of HTR3A mRNA expression in wild-type C57BL/6 mice and B-hHTR3A mice by RT-PCR. brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hHTR3A mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human HTR3A primers. Mouse Htr3a mRNA were detectable only in wild-type C57BL/6 mice. Human HTR3A mRNA was detectable only in homozygous B-hHTR3A mice but not in wild-type mice.
010-56967666（总机） 010-56967680（产品/技术咨询）
投资者关系联络
010-56967601
ir@bbctg.com.cn
京公网安备：