• Origin: The MC38 cell line is derived from C57BL/6 murine colon adenocarcinoma cells. The cell line is a commonly used murine model for colorectal carcinoma.
• Background Information: Seizure protein 6 homolog is a protein that in humans is encoded by the SEZ6 gene. The protein encoded by this gene is thought to contain five cysteine-rich motifs that are similar to sushi domains, as well as two domains similar to the amino terminal half of the CUB (for complement C1r/C1s, Uegf, Bmp1) domain. Mutations in this gene have been associated with febrile seizures.
• Gene targeting strategy: The human SEZ6 coding sequence was inserted into the exon 2 of mouse Sez6 in B-hSEZ6 MC38 cells. Human SEZ6 is highly expressed in the B-hSEZ6 MC38 cells.
• Application: B-hSEZ6 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

SEZ6 expression analysis in B-hSEZ6 MC38 by flow cytometry. Single cell suspensions from wild-type MC38 and B-hSEZ6 MC38 cultures were stained with anti-HLA-SEZ6 antibody. Human SEZ6 was detected in the B-hSEZ6 MC38 cells but not wild-type MC38 cells. The 1-E03 clone of B-HLA-hSEZ6 MC38 cells was used for in vivo experiments.

Subcutaneous homograft tumor growth of B-hSEZ6 MC38 cells. B-hSEZ6 MC38 cells (1x10⁶) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6).  Tumor volume and body weight were measured three times a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hSEZ6 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.