• Origin: The NCI-H929 cell line is a well-established model for multiple myeloma research. It was isolated from the malignant pleural effusion of a 62-year-old female patient with plasma cell myeloma.
• Background Information: Luciferase-expressing cell lines are genetically engineered to produce luciferase enzymes, typically from fireflies (Photinus pyralis) or sea pansies (Renilla reniformis). These bioluminescent reporter systems enable real-time monitoring of cellular processes through light emission when exposed to substrate luciferin. Widely used in molecular biology, they facilitate gene expression studies, drug screening, and in vivo imaging applications. EGFP (Enhanced Green Fluorescent Protein) is a bright, stable variant of the original GFP from jellyfish, widely used as a fluorescent marker in molecular and cellular biology.
• Gene targeting strategy: The CMV promoter and luciferase-EGFP coding sequence were inserted into the targeting vector used in the lentivirus system.
• Tumorigenicity: Confirmed in B-NDG mice.
• Application: Luciferase-expressing B-Tg(Luc-EGFP) NCI-H929 cells are designed for in vivo bioluminescence imaging to monitor tumor progression and evaluate therapeutic efficacy in multiple myeloma models.

Luminescence signal intensity of B-Tg(Luc-EGFP) NCI-H929 cells. Cell lysates of wild-type NCI-H929 and B-Tg(Luc-EGFP) NCI-H929 were measured using the Bright-Glo^TM luciferase Assay (Promega, Catalog No. E4030). B-Tg(Luc-EGFP) NCI-H929 cells have a strong luminescence signal that is not present in wild-type NCI-H929 cells.

Growth kinetics of B-Tg(Luc-EGFP) NCI-H929 tumors determined by bioluminescence imaging (BLI) . B-Tg(Luc-EGFP) NCI-H929 cells (5×10⁶) were injected into the tail vein of wild-type B-NDG mice (female, n=6). Signal intensity and body weight were measured twice a week. (A) Signal intensity. (B) Body weight. (C) Raw bioluminescence images. These results indicate that B-Tg(Luc-EGFP) NCI-H929 cells can be used for in vivo efficacy evaluation. Values are expressed as mean ± SEM.