• Sodium channel subtypes have been linked to human pain syndromes. Nav1.7 is encoded by SCN9A, which plays a critical role in pain sensation in humans, and is predominantly expressed in the dorsal root ganglion (DRG) neurons and sympathetic ganglion neurons. Gain of function mutations in Nav1.7 can cause pain, whilst loss of function Nav1.7 mutations lead to loss of pain in otherwise normal people. There is a great future for sodium channel antagonists, particularly Nav1.7 antagonists in treating most pain syndromes.
• Gene editing strategy: The exons 2-27 of mouse Scn9a gene that encode the whole molecule (ATG to STOP codon) were replaced by human counterparts in B-hSCN9A mice. The promoter, 5’UTR and 3’UTR region of the mouse gene are retained. The human SCN9A expression is driven by endogenous mouse Scn9a promoter, while mouse Scn9a gene transcription and translation will be disrupted.
• mRNA expression analysis: Human SCN9A mRNA was detectable only in homozygous B-hSCN9A mice but not in wild-type mice. Mouse Scn9a mRNA was detectable only in wild-type C57BL/6JNifdc mice.
• Protein expression analysis: SCN9A protein was detected in DRG, brain and cerebellum of both homozygous B-hSCN9A mice and wild-type mice.
• Application: This product is used for the efficacy research and safety evaluation of analgesic drugs, which targeting SCN9A

Gene targeting strategy for B-hSCN9A mice. The exons 2-27 of mouse Scn9a gene that encode the whole molecule (ATG to STOP codon) were replaced by human counterparts in B-hSCN9A mice. The promoter, 5’UTR and 3’UTR region of the mouse gene are retained. The human SCN9A expression is driven by endogenous mouse Scn9a promoter, while mouse Scn9a gene transcription and translation will be disrupted.

Strain specific analysis of SCN9A mRNA expression in wild-type C57BL/6JNifdc mice and homozygous B-hSCN9A mice by RT-PCR. Dorsal root ganglia (DRG) RNA was isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSCN9A mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SCN9A primers. Human SCN9A mRNA was detectable only in homozygous B-hSCN9A mice but not in wild-type mice. Mouse Scn9a mRNA was detectable only in wild-type C57BL/6JNifdc mice.

Western blot analysis of SCN9A protein expression in wild-type C57BL/6JNifdc mice and homozygous B-hSCN9A mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSCN9A mice (H/H), and then analyzed by western blot with anti-Nav1.7 (SCN9A) Antibody. 40 μg total proteins were loaded for western blotting analysis. SCN9A protein was detected in DRG, brain and cerebellum of both homozygous B-hSCN9A mice and wild-type mice.