Gene targeting strategy for B-hLRRC15 mice. The exon 2 of mouse Lrrc15 gene that encodes the extracellular region was replaced by human LRRC15 exon 2 in B-hLRRC15 mice.

Strain specific analysis of LRRC15 gene expression in wild-type C57BL/6 mice and B-hLRRC15 mice by RT-PCR. Mouse Lrrc15 mRNA was detectable only in brain of wild-type C57BL/6 mice (+/+). Human LRRC15 mRNA was detectable only in homozygous B-hLRRC15 mice (H/H), but not in wild-type mice.

Immunohistochemical (IHC) analysis of LRRC15 expression in B-hLRRC15 mice. The brain, lymph node, bone marrow, skin, small intestine and tongue were collected from wild-type C57BL/6JNifdc mice and B-hLRRC15 mice (female, 6-7-week-old), analyzed by IHC with anti-LRRC15 (CST, #50546S). LRRC15 was detectable in these two strains owing to antibody cross-reactivity between human and mouse. LRRC15 was detectable in skin and tongue of B-hLRRC15 mice, but not in brain, lymph node, bone marrow and small intestine. The arrow indicates tissue cells with positive LRRC15 staining (brown). “+” indicates that the tissue is positive, and “-” indicates that the tissue is negative.