• IL1R1 encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. IL1R1 plays a key role in the immune system and is an important research target in tumors, autoimmune diseases, and central nervous system disorders.
• Gene targeting strategy for B-hIL1R1 mice. A chimeric CDS that encodes human IL1R1 signal peptide and extracellular domain, mouse Il1r1 transmembrane and cytoplasmic domain is inserted right after mouse Il1r1 exon 2 to replace the part of exon 2 to exon 5 of mouse Il1r1 gene. The chimeric IL1R1 protein expression will be driven by endogenous mouse Il1r1 promoter, while mouse Il1r1 gene transcription and translation will be disrupted.
• Human IL1R1 mRNA was detectable only in homozygous B-hIL1R1 mice. Human IL1R1 was exclusively detectable in homozygous B-hIL1R1 mice. And IL1B can trigger downstream signaling pathways in B-hIL1R1 mice.

Gene targeting strategy for B-hIL1R1 mice. A chimeric CDS that encodes human IL1R1 signal peptide and extracellular domain, mouse Il1r1 transmembrane and cytoplasmic domain is inserted right after mouse Il1r1 exon 2 to replace the part of exon 2 to exon 5 of mouse Il1r1 gene. The chimeric IL1R1 protein expression will be driven by endogenous mouse Il1r1 promoter, while mouse Il1r1 gene transcription and translation will be disrupted.

Species specific analysis of IL1R1 gene expression in wild-type C57BL/6 mice and homozygous humanized B-hIL1R1 mice by RT-PCR. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL1R1 mice (H/H). Mouse Il1r1 mRNA was detectable only in wild-type C57BL/6 mice. Human IL1R1 mRNA was detectable only in homozygous B-hIL1R1 mice, but not in wild-type C57BL/6 mice.

Strain specific IL1R1 expression analysis in wild-type C57BL/6 mice and homozygous B-hIL1R1 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL1R1 mice (H/H), and cultured in 6-well plates stimulated with GM-CSF and IL-4 for 7 days to induce BMDCs. Then BMDCs were collected and analyzed by flow cytometry with anti-hIL1R1 antibody (R&D, FAB269A-25). Human IL1R1 was exclusively detectable in homozygous B-hIL1R1 mice.

Function analysis of IL1R1 in wild-type C57BL/6 mice and homozygous B-hIL1R1 mice by ELISA. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL1R1 mice (H/H), and then stimulated with 100 ng/mL mouse IL1B (mIL1B) or human IL1B (hIL1B) for 48 h. Both mIL1B and hIL1B can induce mouse IL-6 production in wild-type C57BL/6 mice and homozygous B-hIL1R1 mice. The results indicate that IL1B can trigger downstream signaling pathways in B-hIL1R1 mice.

Note: This experiment is a collaboration with the client

Function analysis of IL1R1 in homozygous B-hIL1R1 mice by ELISA. Plasma was collected from homozygous B-hIL1R1 mice (H/H) stimulated with human IL-1β (hIL-1β, i.p., 200 μL/mouse) in vivo for 2 hours and 6 hours. Expression level of hIL-1β, mouse CXCL2, CCL2, IL-6, G-CSF and CCL3 were detected and analyzed. hIL-1β can promote the production of mouse CXCL1, CCL2, IL-6, G-CSF and CCL3 in a short period in homozygous B-hIL1R1 mice. The results indicate that IL-1β protein can effectively activate its downstream signaling pathway and trigger systemic inflammatory responses in a short period of time.

Note: This experiment is a collaboration with the client

Function analysis of IL1R1 in homozygous B-hIL1R1 mice by ELISA. Splenocytes were collected from homozygous B-hIL1R1 mice (H/H) incubated with increasing doses of Anakirina (commercially purchased) for an hour and then stimulated with human IL-1β (hIL-1β) (0.2 ng/mL) in vitro for 48 hours. The supernatant was collected, following with the detection of mouse IL-6 expression by ELISA. The result showed that splenocytes from B-hIL1R1 mice responded well to hIL-1β with mouse IL-6 production. Anakirina (commercially purchased) could effectively reduce the expression of mouse IL-6 to the same level of vehicle-treated group, indicating a successful inhibition of the downstream activation caused by hIL1β.

Note: This experiment is a collaboration with the client