Gene targeting strategy for B-hNGAL mice. The exons 1~6 of mouse Ngal gene that encode the full-length protein were replaced by human NGAL exons 1~6 in B-hNGAL mice.

Strain specific NGAL expression analysis in homozygous B-hNGAL mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hNGAL mice (H/H) and analyzed by ELISA with species-specific NGAL ELISA kit. Mouse NGAL was detectable in wild-type mice (A). Human NGAL was exclusively detectable in homozygous B-hNGAL mice but not in wild-type mice (B).

Strain specific analysis of NGAL gene expression in wild-type C57BL/6JNifdc mice and B-hNGAL mice by RT-qPCR. Various tissue RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) (male, 7w, n=3) and homozygous B-hNGAL mice (H/H) (male, 7w, n=3). Values are expressed as mean ± SEM.

Strain specific analysis of NGAL mRNA expression in wild-type C57BL/6JNifdc and B-hNGAL mice by RT-PCR. Various tissue RNA were isolated from wild-type C57BL/6JNifdc (+/+) and homozygous B-hNGAL mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human NGAL primers. Human NGAL mRNA was exclusively detectable in homozygous B-hNGAL mice.

Strain specific analysis of NGAL mRNA expression in wild-type C57BL/6JNifdc and B-hNGAL mice by RT-PCR. Various tissue RNA were isolated from wild-type C57BL/6JNifdc (+/+) and homozygous B-hNGAL mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human NGAL primers. Human NGAL mRNA was exclusively detectable in homozygous B-hNGAL mice.