B-hEFEMP1 MC38

• 321926

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B-hEFEMP1 MC38

Product nameB-hEFEMP1 MC38
Catalog number321926
Strain backgroundC57BL/6
NCBI gene ID2202 (Human)
AliasesEFEMP1
TissueColon
DiseaseColon carcinoma
SpeciesMouse
ApplicationB-hEFEMP1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
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  • Description
  • Targeting strategy
  • Phenotypic analysis

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      Description

      The mouse Efemp1 gene was replaced by human EFEMP1 coding sequence in B-hEFEMP1 MC38 cells. Human EFEMP1 is highly expressed on the supernatant of B-hEFEMP1 MC38 cells.

      Tageting strategy
      Gene targeting strategy for B-hEFEMP1 MC38 cells. The exogenous promoter and human EFEMP1 coding sequence was inserted to replace part of murine exon 4. The insertion disrupts the endogenous murine Efemp1 gene, resulting in a non-functional transcript.
      Protein expression analysis

      EFEMP1 expression analysis in B-hEFEMP1 MC38 cells by western blot. Human EFEMP1 was detected in the supernatant of B-hEFEMP1 MC38 cells but not wild-type MC38 cells. The 2-F12 clone of B-hEFEMP1 MC38 cells was used for in vivo experiments.

      Tumor growth curve & Body weight changes

      Subcutaneous homograft tumor growth of B-hEFEMP1 MC38 cells. B-hEFEMP1 MC38 cells (5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hEFEMP1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

      Protein expression analysis
      B-hEFEMP1 MC38 tumor cells growth of individual mice. B-hEFEMP1 MC38 cells (5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=6). As shown in panel, B-hEFEMP1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.