B-hAREG mice

C57BL/6N-Aregtm1(AREG)Bcgen/Bcgen • 111615

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B-hAREG mice

Product nameB-hAREG mice
Catalog number111615
Strain nameC57BL/6N-Aregtm1(AREG)Bcgen/Bcgen
Strain backgroundC57BL/6N
NCBI gene ID374 (Human)
AliasesARB, CRDGF, SDGF, AREG
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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hAREG mice. The exons 2~4 of mouse Areg gene were replaced by human AREG exons 2~4 in B-hAREG mice.

      mRNA expression analysis
      Strain specific analysis of AREG mRNA expression in wild-type C57BL/6 mice and B-hAREG mice by RT-PCR. Stomach RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hAREG mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human AREG primers. Mouse Areg mRNA was detectable only in wild-type C57BL/6 mice. Human AREG mRNA was detectable only in homozygous B-hAREG mice but not in wild-type mice.
      Protein expression analysis

      Western blot analysis of AREG protein expression in homozygous B-hAREG mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hAREG mice (H/H), and then analyzed by western blot with anti-AREG antibody. 30μg total proteins were loaded for western blotting analysis. AREG protein was detected in the testis, brain, lung, stomach of wild-type C57BL/6 mice and homozygous B-hAREG mice, as the antibody is cross-recognize both human and mouse AREG.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hAREG mice] (Cat# 111615) was purchased from Biocytogen.