• Background: Complement component C3 is a 190 kDa protein that is the most prevalent complement protein in serum and is involved in the production of fragments C3a and C3b, which are crucial for complement activation and immune response. It is also necessary for all three complement-activating pathways: classical, lectin, and alternative.
• Validation:

1. Rat C3 was only detectable in wild-type SD rats but not in B-hC3*R102G rats. Human C3 was exclusively detectable in B-hC3*R102G rats but not in wild-type SD rats.
2. C3 was detected in the brain, liver, eye, and kidney of B-hC3*R102G rats and wild-type SD rats by IHC staining.
3. There were no obvious abnormalities found in 10 weeks old wild-type SD rats and B-hC3*R102G rats.

Strain-specific human C3 expression analysis in wild-type SD rats and B-hC3*R102G rats by ELISA. Serum was collected from wild-type SD rats (+/+) (male, female, n=3, 10 weeks old) and B-hC3*R102G rats (H/H) (male, female, n=2-3, 10 weeks old). The expression level of rat C3 was analyzed by ELISA (Abcam, ab157731). The expression level of human C3 was analyzed by ELISA (Abcam, ab108823). Rat C3 was only detectable in wild-type SD rats but not in B-hC3*R102G rats. Human C3 was exclusively detectable in B-hC3*R102G rats but not in wild-type SD rats.

Histopathological analysis of kidneys in B-hC3*R102G rats. The kidney tissues of wild-type SD rats (+/+) and B-hC3*R102G rats (H/H) were isolated at 10 weeks of age and analyzed with H&E staining (male, n=1). There were no obvious abnormalities found in wild-type SD rats (+/+) and B-hC3*R102G rats (H/H). Scale bar: 200 μm.

IHC Staining in B-hC3*R102G rats. The brain, liver, kidney, and eye tissues of wild-type SD rats (+/+) and B-hC3*R102G rats (H/H) were isolated at 10 weeks of age and analyzed with IHC staining (male, n=1). Results showed that C3 was detected in the brain, liver, eye, and kidney of B-hC3*R102G rats and wild-type SD rats, as the C3 antibody is cross-recognizing both human and rats (Abcam, ab200999).