C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfr2tm1(TNFR2)Bcgen/Bcgen • 111843
| Product name | B-hCTLA4/hTNFR2 mice |
|---|---|
| Catalog number | 111843 |
| Strain name | C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfr2tm1(TNFR2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 1493,7133 (Human) |
| Aliases | ALPS5; CD; CD152; CELIAC3; CTLA-4; GRD4; GSE; IDDM12; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; p75TNFR; IDDM12; CD120b |
Gene targeting strategy for B-hCTLA4/hTNFR2 mice. The exon 2 of mouse Ctla4 gene that encodes the extracellular domain was replaced by human CTLA4 exon 2 in B-hCTLA4/hTNFR2 mice. The exons 2~6 of mouse Tnfr2 gene that encode the extracellular domain was replaced by human TNFR2 exons 2~6 in B-hCTLA4/hTNFR2 mice.
Strain specific hCTLA4 expression analysis in homozygous B-hCTLA4/hTNFR2 mice by flow cytometry. Spleen was collected from wild-type mice (+/+) and homozygous B-hCTLA4/hTNFR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD152 (CTLA4) antibodies. Mouse CTLA4 was detectable in wild-type mice. Human CTLA4 was exclusively detectable in homozygous B-hCTLA4/hTNFR2 mice but not in wild-type mice.
Strain specific hTNFR2 expression analysis in homozygous B-hCTLA4/hTNFR2 mice by flow cytometry. Spleen was collected from wild-type mice (+/+) and homozygous B-hCTLA4/hTNFR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-TNFR2 antibodies. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hCTLA4/hTNFR2 mice but not in wild-type mice.
