B-hIL36A mice

C57BL/6N-Il36atm1(IL36A)Bcgen/Bcgen • 110092

订购产品
B-hIL33/hTSLP/hTSLPR mice plus
B-hIL36R mice

B-hIL36A mice

Product nameB-hIL36A mice
Catalog number110092
Strain nameC57BL/6N-Il36atm1(IL36A)Bcgen/Bcgen
Strain backgroundC57BL/6N
NCBI gene ID27179 (Human)
AliasesFIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6
订购产品

在此页面上

  • Targeting strategy
  • Phenotypic analysis
  • Related products

海报

查看全部

    发表文章

      相关产品

      查看全部
      Tageting strategy

      Gene targeting strategy for B-hIL36A mice.

      The exon 2-5 of mouse Il36a gene that encodes the full-length protein were replaced by human IL36A exon 1-4 in B-hIL36A mice.

      mRNA expression analysis

      Strain specific analysis of IL36A gene expression in wild type (WT) mice and B-hIL36A mice by RT-PCR. Mouse Il36a mRNA was detectable only in stomach of WT mice (+/+). Human IL36A mRNA was detectable only in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).

      Protein expression analysis
      Strain specific IL36A expression analysis in homozygous B-hIL36A mice by western blot . Skin were collected from wild type (WT) mice (+/+) and homozygous B-hIL36A mice (H/H), and analyzed by western blot with anti-IL36A antibody. Mouse IL36A was detectable in WT mice (+/+) due to the cross-reactivity of antibodies. Human IL36A was detectable in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).
      Product list