Targeting strategy
Gene targeting strategy for B-hIL36A mice.
The exon 2-5 of mouse Il36a gene that encodes the full-length protein were replaced by human IL36A exon 1-4 in B-hIL36A mice.
mRNA expression analysis
Strain specific analysis of IL36A gene expression in wild type (WT) mice and B-hIL36A mice by RT-PCR. Mouse Il36a mRNA was detectable only in stomach of WT mice (+/+). Human IL36A mRNA was detectable only in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific IL36A expression analysis in homozygous B-hIL36A mice by western blot .
Skin were collected from wild type (WT) mice (+/+) and homozygous B-hIL36A mice (H/H), and analyzed by western blot with anti-IL36A antibody. Mouse IL36A was detectable in WT mice (+/+) due to the cross-reactivity of antibodies. Human IL36A was detectable in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hIL36A mice] (Cat# 110092) was purchased from Biocytogen.
