Description
- Heat Shock Protein 47 (HSP47), also known as Serpin-H1, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.
- In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen, which is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.
- The exons 1-5 of mouse Serpinh1 gene that encode the whole molecule (ATG to STOP codon), including promoter, 5’UTR and 3’UTR were replaced by human counterparts in B-hSERPINH1 mice. The human SERPINH1 expression is driven by human Serpinh1 promoter, while mouse Serpinh1 gene transcription and translation will be disrupted.
- SERPINH1 protein was detectable in in heart, liver(weak expression), lung(weak expression), kidney(weak expression), uterus and ovary of wild-type C57BL/6 mice and homozygous B-hSERPINH1 mice, as the antibody can cross-recognize both human and mouse SERPINH1.
- Mouse Serpinh1 mRNA was detectable in wild-type C57BL/6 mice. Human SERPINH1 mRNA was detectable in homozygous B-hSERPINH1 mice.
Targeting strategy
Gene targeting strategy for B-hSERPINH1 mice. The exons 1-5 of mouse Serpinh1 gene that encode the whole molecule (ATG to STOP codon), including promoter, 5’UTR and 3’UTR were replaced by human counterparts in B-hSERPINH1 mice. The human SERPINH1 expression is driven by human SERPINH1 promoter, while mouse Serpinh1 gene transcription and translation will be disrupted.
mRNA expression analysis in humanized B-hSERPINH1 mice
Species specific analysis of SERPINH1 gene expression in wild-type C57BL/6 mice and homozygous humanized B-hSERPINH1 mice by RT-PCR. Lung was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSERPINH1 mice (H/H). Mouse Serpinh1 mRNA was detectable in wild-type C57BL/6 mice. Human SERPINH1 mRNA was detectable only in homozygous B-hSERPINH1 mice, but not in wild-type C57BL/6 mice.
Protein expression analysis
Strain specific SERPINH1 expression analysis in homozygous B-hSERPNH1 mice by western blot. Heart, liver, lung, kidney, uterus and ovary were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSERPINH1 mice (H/H), and analyzed by western blot with anti-SERPINH1 antibody (proteintech, 10875-1-AP). m/hSERPINH1 was detectable in heart, liver(weak expression), lung(weak expression), kidney(weak expression), uterus and ovary of wild-type C57BL/6 mice and homozygous B-hSERPINH1 mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hSERPINH1 mice] (Cat# 112961) was purchased from Biocytogen.
