C57BL/6-Tnftm1(TNF)Bcgen Tnfrsf1atm1(TNFRSF1A)Bcgen Tnfrsf1btm1(TNFRSF1B)Bcgen /Il6tm1(IL6)Bcgen Il6rtm2(IL6R)Bcgen/Bcgen • 114254
| Product name | B-hTNFA/hTNFR2/hTNFR1/hIL6/hIL6R mice |
|---|---|
| Catalog number | 114254 |
| Strain name | C57BL/6-Tnftm1(TNF)Bcgen Tnfrsf1atm1(TNFRSF1A)Bcgen Tnfrsf1btm1(TNFRSF1B)Bcgen /Il6tm1(IL6)Bcgen Il6rtm2(IL6R)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 7124,7132,7133,3569,3570 (Human) |
| Aliases | DIF; TNFA; IMD127; TNFSF2; TNLG1F; TNF-alpha; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2; IL6Q; gp80; CD126; HIES5; IL-6R; IL6RA; IL6RQ; IL-1Ra; IL-6RA; IL6QTL; IL-6R-1 |
Gene targeting strategy for B-hTNFA/hTNFR2/hTNFR1/hIL6/hIL6R mice.
Human TNFA, human IL6, and mouse IFNγ expression analysis in homozygous humanized B-hTNFA/hTNFR2/hTNFR1/hIL6/hIL6R mice by ELISA. Mice received LPS injection (i.p.) two hours following PBS or adalimumab analog (i.v., in house) treatment. Serum was collected from homozygous B-hTNFA/hTNFR2/hTNFR1/hIL6/hIL6R mice one hour and four hours after LPS injection. Expression level of human TNFA, human IL6, and mouse IFNγ, were analyzed by ELISA (ELISA MAX™ Deluxe Set Human TNF-α, Biolegend, 430204; ELISA MAX™ Deluxe Set Human IL-6, Biolegend, 430508; ELISA MAX™ Deluxe Set Mouse IFN-γ, Biolegend, 430804). Human TNFA level was significantly increased one hour and four hours after LPS injection. Human IL6 level was significantly increased one and four hours after LPS injection. Mouse IFNγ level was significantly increased four hours after LPS injection. Pre-treatment of adalimumab analog (in house) significantly decreased human TNFA expression at one hour after LPS injection, human IL6 one and four hours after LPS stimulation, and mouse IFNγ expression at four hours after LPS injection, indicating a successful inhibition of the downstream activation caused by hTNFA. Values are expressed as mean ± SEM. Significance was determined by one-way ANOVA test . * p < 0.5, **p < 0.01, ***p < 0.001.