Gene targeting strategy for B-hNCR3 mice(CB-17 SCID). The full coding sequences of human NCR3 gene, including the promoter, 5’UTR and 3’UTR are inserted into mouse Hipp11 (H11) locus in B-hNCR3 mice(CB-17 SCID).

Strain specific NCR3 expression analysis with blood from homozygous B-hNCR3 mice(CB-17 SCID) by flow cytometry. Blood cells were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hNCR3 mice(CB-17 SCID) (H/H), and analyzed by flow cytometry with species-specific anti-human NCR3 antibody (Biolegend, 325208). Human NCR3 was exclusively detectable in homozygous B-hNCR3 mice(CB-17 SCID).

Strain specific NCR3 expression analysis with splenocytes from homozygous B-hNCR3 mice(CB-17 SCID) by flow cytometry. Splenocytes were collected from wild-type CB-17 SCID mice (+/+) and homozygous B-hNCR3 mice(CB-17 SCID) (H/H), and analyzed by flow cytometry with species-specific anti-human NCR3 antibody (Biolegend, 325208). Human NCR3 was exclusively detectable in homozygous B-hNCR3 mice(CB-17 SCID).