Description
The IL17A is a pro-inflammatory cytokine that, although expressed by several cell types, defines the T helper 17 (TH17) cell subset. IL17A activates signal transduction pathways that recruit neutrophils, promote the production of antimicrobial factors, and drive the inflammatory pathology associated with autoimmune disorders.
Targeting strategy
The Il17aEGFP mice were developed by Beijing Biocytogen Co., Ltd. An IRES-EGFP-SV40-polyA signal sequence cassette was inserted after the stop codon of the Il17a gene using C57BL/6-derived embryonic stem (ES) cells. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
Fluorescence can be observed in some intraepithelial lymphocytes (IELs) of the small intestine without stimulation. Naive CD4+ T cells stimulated with plate-bound anti-CD3/anti-CD28 in the presence of transforming growth factor, beta 1 (TGFB, 1 ng/ml) and interleukin 6 (IL6, 20 ng/ml) show increased fluorescence associated with IL17A production. Normal levels of IL17A are expressed.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-IL17a-EGFP mice] (Cat# 110161) was purchased from Biocytogen.
