B-hADAM9 mice

C57BL/6-Adam9tm1(ADAM9)Bcgen/Bcgen • 111342

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B-hADAM9 mice

Product nameB-hADAM9 mice
Catalog number111342
Strain nameC57BL/6-Adam9tm1(ADAM9)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID8754 (Human)
AliasesCORD9, MCMP, MDC9, Mltng
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  • Phenotypic analysis

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      Protein expression analysis

      Western blot analysis of ADAM9 protein expression in homozygous B-hADAM9 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hADAM9 mice (H/H), and then analyzed by western blot with anti-ADAM9 antibody. 40 μg total proteins were loaded for western blotting analysis. ADAM9 protein was detected in the heart, liver, spleen, lung, kidney, brain and thymus of wild-type C57BL/6 mice and homozygous B-hADAM9 mice, as the antibody is cross-recognize both human and mouse ADAM9.

      mRNA expression analysis
      Strain specific analysis of ADAM9 mRNA expression in wild-type C57BL/6 mice and B-hADAM9 mice by RT-PCR. Brain and lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADAM9 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADAM9 primers. Mouse Adam9 mRNA was detectable only in wild-type C57BL/6 mice. Human ADAM9 mRNA was detectable only in homozygous B-hADAM9 mice but not in wild-type mice. And the PCR products were confirmed with sanger sequencing.