C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)BcgenCd3gtm1(CD3G)Bcgen Tnfrsf17tm1(TNFRSF17)Bcgen/Bcgen • 112602
| Product name | B-hCD3EDG/hBCMA mice |
|---|---|
| Catalog number | 112602 |
| Strain name | C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)BcgenCd3gtm1(CD3G)Bcgen Tnfrsf17tm1(TNFRSF17)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 916,915,917,608 (Human) |
| Aliases | IMD18, T3E, TCRE; CD3-DELTA, IMD19, T3D; CD3-GAMMA, IMD17, T3G; BCM, BCMA, CD269, TNFRSF13A |
Targeting strategyGene targeting strategy for B-hCD3EDG/hBCMA mice.
The exons 2-8 of mouse Cd3e gene that encode the full-length protein were replaced by human CD3E exons 2-9 in B-hCD3EDG/hBCMA mice. The exons 1-5 of mouse Cd3d and the exons 1-6 of Cd3g gene that encode the full-length protein were replaced by human CD3D exons 1-5 and CD3G exons 1-7 in B-hCD3EDG/hBCMA mice, respectively. The exon 1 and part of exon 2 encode the extracellular region of mouse Bcma gene were replaced with human BCMA exon 1 and part of exon 2 inB-hCD3EDG/hBCMA mice
mRNA expression analysis
Strain specific analysis of CD3EDG and BCMA gene expression in wild type (WT) mice and B-hCD3EDG/hBCMA mice by RT-PCR.
Mouse Cd3edg and Bcma mRNA were detectable only in splenocytes of WT mice (+/+). Human CD3EDG and BCMA mRNA were detectable only in homozygous B-hCD3EDG/hBCMA mice (H/H; H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing.
Protein expression analysis
Strain specific CD3E expression analysis in homozygous B-hCD3EDG/hBCMA mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hBCMA mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hBCMA mice (H/H; H/H) but not in WT mice (+/+).
