C57BL/6N-F11rtm1(F11R)Bcgen/Bcgen • 112164
Targeting strategyGene targeting strategy for B-hF11R mice. The exons 2-7 of mouse F11r gene that encode the extracellular domain were replaced by human F11R exons 2-7 in B-hF11R mice.
Protein expression analysis of F11R(JAM1) in platelet
Strain specific F11R expression analysis in homozygous B-hF11R mice by flow cytometry. Platelets were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF11R mice (H/H), and analyzed by flow cytometry with species-specific anti-F11R antibody. Mouse F11R was detectable in platelet of wild-type C57BL/6 mice. Human F11R was exclusively detectable in platelet of homozygous B-hF11R mice but not in wild-type C57BL/6 mice.
Protein expression analysis of F11R(JAM1) in T cells, B cells and Monocytes of splenocytes
Strain specific F11R expression analysis in homozygous B-hF11R mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF11R mice (H/H), and analyzed by flow cytometry with species-specific anti-F11R antibodies. Mouse F11R was not detectable in T cells (A), B cells (B) and monocytes (C) of wild-type C57BL/6 mice. Human F11R was not detectable in T cells (A), B cells (B) and was detectable monocytes (C) of homozygous B-hF11R mice.
Protein expression analysis of F11R(JAM1) in NK cells, dendritic cells and Macrophages of splenocytes
Strain specific F11R expression analysis in homozygous B-hF11R mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF11R mice (H/H), and analyzed by flow cytometry with species-specific anti-F11R antibodies. Mouse F11R was detectable in NK cells (A), dendritic cells (B) and macrophages (C) of wild-type C57BL/6 mice. Human F11R was exclusively detectable in NK cells (A), dendritic cells (B) and macrophages (C) of homozygous B-hF11R mice but not in wild-type C57BL/6 mice.
Protein expression analysis of F11R(JAM1) in Stem cells of bone marrow
Strain specific F11R expression analysis in homozygous B-hF11R mice by flow cytometry. Bone marrow were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF11R mice (H/H), and analyzed by flow cytometry with species-specific anti-F11R antibodies. Mouse F11R was detectable in stem cells of wild-type C57BL/6 mice. Human F11R was exclusively detectable in stem cells of homozygous B-hF11R mice but not in wild-type C57BL/6 mice.
Protein expression analysis of F11R(JAM1) in Endothelial cells of lung
Strain specific F11R expression analysis in homozygous B-hF11R mice by flow cytometry. Lung were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF11R mice (H/H), and analyzed by flow cytometry with species-specific anti-F11R antibodies. Mouse F11R was detectable in endothelial cells of wild-type C57BL/6 mice. Human F11R was exclusively detectable in endothelial cells of homozygous B-hF11R mice but not in wild-type C57BL/6 mice.
