B-hHBEGF mice

C57BL/6N-Hbegftm1(HBEGF)Bcgen/Bcgen • 111089

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B-hHBEGF mice

Product nameB-hHBEGF mice
Catalog number111089
Strain nameC57BL/6N-Hbegftm1(HBEGF)Bcgen/Bcgen
Strain backgroundC57BL/6N
NCBI gene ID1839 (Human)
AliasesDTR; DTS; DTSF; HEGFL
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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hHBEGF mice. The exons 2~4 of mouse Hbegf gene that encode the extracellular domain were replaced by human HBEGF exons 2~4 in B-hHBEGF mice.

      Protein expression analysis

      Western blot analysis of HBEGF protein expression in homozygous B-hHBEGF mice. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and homozygous B-hHBEGF mice (H/H), and then analyzed by western blot with species-specific anti-HBEGF antibody. 40 μg total proteins were loaded for western blotting analysis. Pro-HBEGF and soluble HBEGF (sHBEGF) were detected in brain, lung, kidney, liver and spleen. The antibodies showed cross-recognition between human and mouse.

      mRNA expression analysis
      Strain specific analysis of HBEGF mRNA expression in wild-type C57BL/6N mice and B-hHBEGF mice by RT-PCR. Heart and lung RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hHBEGF mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse and human HBEGF primers. As the coding region is only about 630bp, the same primers were used to amplify both mouse Hbegf and human HBEGF gene. PCR products in heart and lung for human HBEGF were confirmed via Sanger Sequencing.
      sHBEGF Protein Expression Analysis

      Protein expression analysis of human HBEGF in homozygous B-hHBEGF mice by ELISA. Various tissue homogenates were collected from wild-type C57BL/6N mice (male, n=3, 7-week-old) and homozygous B-hHBEGF mice (male, n=3, 7-week-old). Protein expression level of human HBEGF was analyzed by ELISA with anti-human HBEGF antibody (R&D, DHBEG0). This ELISA kit has less cross-reactivity with mouse HBEGF. Human sHBEGF was detectable in homozygous B-hHBEGF mice.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hHBEGF mice] (Cat# 111089) was purchased from Biocytogen.