Gene targeting strategy for B-hIBSP mice. The exons 2~7 of mouse Ibsp gene that encodes the full-length protein was replaced by human IBSP exons 2~7 in B-hIBSP mice.

Strain specific IBSP expression analysis in homozygous B-hIBSP mice by ELISA. Serum was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hIBSP mice (H/H) and analyzed by ELISA with IBSP ELISA kit. Mouse IBSP was detectable in wild-type mice and homozygous B-hIBSP mice as the kit has partly cross reactions with human and mouse IBSP (A). Human IBSP was exclusively detectable in homozygous B-hIBSP mice but not in wild-type mice (B).