Gene targeting strategy for B-hIGFBP2 mice. The exons 1-4 of mouse Igfbp2 gene that encode the full-length protein were replaced by human IGFBP2 exons 1-4 in B-hIGFBP2 mice.

Strain specific IGFBP2 expression analysis in heterozygous B-hIGFBP2 mice by Western Blot. Liver tissue was collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hIGFBP2 mice (H/+) , and analyzed by western blot with species-specific IGFBP2 antibody. Mouse IGFBP2 was detectable in wild type mice and heterozygous B-hIGFBP2 mice, as the antibody is crossly reactive with IGFBP2 in human and mice. Human IGFBP2 was exclusively detectable in heterozygous B-hIGFBP2 mice but not wild-type mice.