C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Il1btm1(IL1B)Bcgen/Bcgen • 131619
| Product name | B-hPD-1/hPD-L1/hIL1B mice |
|---|---|
| Catalog number | 131619 |
| Strain name | C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Il1btm1(IL1B)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 5133,29126,3553 (Human) |
| Aliases | CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1; B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1; IL-1, IL1-BETA, IL1F2, IL1beta |
Protein expression analysis
Strain specific PD-1 and PD-L1 expression analysis in wild-type C57BL/6 mice and homozygous B-hPD-1/hPD-L1/hIL1B mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPD-L1/hIL1B mice (H/H) after stimulated with or without anti-CD3ε in vivo. Mouse PD-1 and PD-L1 were detectable in wild-type C57BL/6 mice. Human PD-1 and PD-L1 were exclusively detectable in homozygous B-hPD-1/hPD-L1/hIL1B mice but not in wild-type mice.
Strain specific IL1B expression analysis in homozygous B-hPD-1/hPD-L1/hIL1B mice by ELISA. Bone marrow cells were collected from C57BL/6 (+/+) and homozygous B-hPD-1/hPD-L1/hIL1B mice (H/H) and stimulated with LPS for 24 hours in vitro. The supernatant was analyzed with species-specific IL1B ELISA kit. Mouse IL1B was detectable in C57BL/6 mice. Human IL1B was exclusively detectable in homozygous B-hPD-1/hPD-L1/hIL1B mice but not C57BL/6 mice. ND, not detectable.
