Protein expression analysis
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry.Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H), and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable in wild-type C57BL/6 mice (+/+). Human PSGL-1 was exclusively detectable in homozygous B-hPSGL-1 mice (H/H) but not wild-type C57BL/6 mice (+/+).
Protein expression analysis-spleen T cells
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on T cells of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on T cells of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-spleen B cells
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry.Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on B cells of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on B cells of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-spleen NK cells
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on NK cells of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on NK cells of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-spleen DCs
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on dendritic cells of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on dendritic cells of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-spleen neutrophils
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on neutrophils of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on neutrophils of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-spleen monocytes
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on monocytes of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on monocytes of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-spleen macrophages
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on macrophages of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on macrophages of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-blood T cells
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on T cells of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on T cells of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-blood B cells
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on B cells of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on B cells of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-blood NK cells
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on NK cells of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on NK cells of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-blood DCs
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on dendritic cells of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on dendritic cells of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-blood neutrophils
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on neutrophils of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on neutrophils of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-blood monocytes
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on monocytes of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on monocytes of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression analysis-blood macrophages
Strain specific PSGL-1 expression analysis in homozygous B-hPSGL-1 mice by flow cytometry. Blood were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPSGL-1 mice (H/H) and analyzed by flow cytometry with species-specific anti-PSGL-1 antibody. Mouse PSGL-1 was detectable on macrophages of wild-type C57BL/6 mice. Human PSGL-1 was detectable only on macrophages of homozygous B-hPSGL-1 mice but not on wild-type mice.
Protein expression
Analysis of Leukocyte Subpopulations
Analysis of leukocyte subpopulations in immune organs and blood by flow cytometry. Splenocytes, peripheral blood, and lymph nodes were isolated from female wild-type C57BL/6N mice and homozygous B-hPSGL-1 mice. Single live cells were gated on the CD45+ population and analyzed by flow cytometry as indicated. Values are expressed as mean ± SEM.
Analysis of T Cell Subpopulations
Analysis of T-cell subpopulations in immune organs and blood by flow cytometry. Splenocytes, peripheral blood, and lymph nodes were isolated from female wild-type C57BL/6N mice and homozygous B-hPSGL-1 mice. Single live cells were gated on the CD3+ T-cell population and analyzed by flow cytometry as indicated. Values are expressed as mean ± SEM.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hPSGL-1 mice] (Cat# 111470) was purchased from Biocytogen.
