Gene targeting strategy for B-hSRB1 mice. The exons 2-11 of mouse Scarb1 gene that encode the extracellular domain and partial transmembrane domain of were replaced by human SCARB1 exons 2-11 in B-hSRB1 mice.

Strain specific analysis of SRB1 gene expression in wild type (WT) mice and B-hSRB1 mice by RT-PCR. Mouse Srb1 mRNA was detectable in liver of WT mice (+/+). Human SRB1 mRNA was exclusively detectable in homozygous B-hSRB1 mice (H/H) but not in WT mice (+/+).

Western blot analysis of SRB1 protein expression in homozygous B-hSRB1 mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSRB1 mice (H/H) (male, 6-week-old), and then analyzed by western blot with anti-SRB1 antibody (abcam, ab217318). 40 μg total proteins were loaded for western blotting analysis. SRB1 was detected in the liver, adrenal glands and testis (at a lower expression) of both wild-type mice and homozygous B-hSRB1 mice, as the antibody used exhibited cross-reactivity with mouse and human SRB1.