C57BL/6-Timd4tm1(TIMD4)Bcgen Havcr1tm1(HAVCR1)Bcgen/Bcgen • 110982
| Product name | B-hTIM4/hTIM1 mice |
|---|---|
| Catalog number | 110982 |
| Strain name | C57BL/6-Timd4tm1(TIMD4)Bcgen Havcr1tm1(HAVCR1)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 91937,26762 (Human) |
| Aliases | SMUCKLER, TIM4; CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1 |
Targeting strategyGene targeting strategy for B-hTIM4/hTIM1 mice. The mouse Tim4 gene that encodes the extracellular and transmembrane regions were replaced by human TIM4 counterpart in B-hTIM4/hTIM1 mice. The mouse Tim1 gene that encode the extracellular domain were replaced by human TIM1 counterpart gene in B-hTIM4/hTIM1 mice.
Protein expression analysis
Strain specific TIM1 expression analysis in homozygous B-hTIM4/hTIM1 mice by flow cytometry. Kidney tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTIM4/hTIM1 mice (H/H) stimulated with folic acid in vivo (5000 μg/mice, stimulation for 22 hours, i.p. ), and analyzed by flow cytometry with species-specific anti-TIM1 antibody. Mouse TIM1 was detectable in wild-type mice. Human TIM1 were exclusively detectable in homozygous B-hTIM4/hTIM1 mice but not in wild-type mice.
