Gene targeting strategy for B-NDG hEPCAM mice. The exons 3-8 of mouse Epcam gene that encode extracellular domain and transmembrane domain is replaced by human counterparts in B-hEPCAM mice. The genomic region of mouse Epcam gene that encodes signal peptide and cytoplasmic portion is retained. The promoter, 5’UTR and 3’UTR region of the mouse gene are also retained. The chimeric EPCAM expression is driven by endogenous mouse Epcam promoter, while mouse Epcam gene transcription and translation will be disrupted.

Strain specific EPCAM expression analysis in homozygous B-NDG hEPCAM mice by flow cytometry. Small intestine, pancreas and lung were collected from wild-type B-NDG mice (+/+) and homozygous B-NDG hEPCAM mice (H/H), and CD45 negative cells were analyzed by flow cytometry with species-specific anti-mouse EPCAM antibody (Biolegend, 118205) and anti-human EPCAM antibody (Biolegend, 369809/324208). Mouse EPCAM was detectable in wild-type B-NDG mice. Human EPCAM was exclusively detectable in homozygous B-NDG hEPCAM mice but not in wild-type mice.