C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)BcgenCd3gtm1(CD3G)Bcgen Tnfrsf17tm1(TNFRSF17)Bcgen/Bcgen • 112602
| Product name | B-hCD3EDG/hBCMA mice |
|---|---|
| Catalog number | 112602 |
| Strain name | C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)BcgenCd3gtm1(CD3G)Bcgen Tnfrsf17tm1(TNFRSF17)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 916,915,917,608 (Human) |
| Aliases | T3E; TCRE; IMD18; CD3epsilon; T3D; IMD19; CD3DELTA; CD3-DELTA; T3G; IMD17; CD3GAMMA; CD3-GAMMA; BCM; BCMA; CD269; TNFRSF13A |
Strain specific analysis of CD3EDG and BCMA gene expression in wild type (WT) mice and B-hCD3EDG/hBCMA mice by RT-PCR.
Mouse Cd3edg and Bcma mRNA were detectable only in splenocytes of WT mice (+/+). Human CD3EDG and BCMA mRNA were detectable only in homozygous B-hCD3EDG/hBCMA mice (H/H; H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing.
Strain specific CD3E expression analysis in homozygous B-hCD3EDG/hBCMA mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hBCMA mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hBCMA mice (H/H; H/H) but not in WT mice (+/+).
BCMA expression analysis in wild-type C57BL/6 mice and homozygous B-hCD3EDG/hBCMA mice by flow cytometry. Spleen plasma cells were collected from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hBCMA mice. Protein expression was analyzed with human BCMA antibody (Biolegend, 357504). Human BCMA was detectable in plasma cells of spleen from B-hCD3EDG/hBCMA mice but not in wild-type C57BL/6 mice.
Note: Both groups of mice were treated using an undisclosed methods.
