B-hPD-1/hPCSK9 mice

C57BL/6-Pdcd1tm1(PDCD1)Bcgen Pcsk9tm1(PCSK9)Bcgen/Bcgen • 111861

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B-hPD-1/hPCSK9 mice

Product nameB-hPD-1/hPCSK9 mice
Catalog number111861
Strain nameC57BL/6-Pdcd1tm1(PDCD1)Bcgen Pcsk9tm1(PCSK9)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID255738,5133 (Human)
AliasesFH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9; CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
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  • Phenotypic analysis

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      mRNA expression analysis
      Strain specific analysis of PCSK9 mRNA expression in wild-type C57BL/6 mice and B-hPD-1/hPCSK9 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPCSK9 mice (H/H;H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human PCSK9 primers. Human PCSK9 mRNA was detectable only in homozygous B-hPD-1/hPCSK9 mice.
      Protein expression analysis
      Strain specific PCSK9 expression analysis in homozygous B-hPD-1/hPCSK9 mice by ELISA. Serum were collected from wild-type mice C57BL/6 mice (+/+) and homozygous B-hPD-1/hPCSK9 mice (H/H;H/H), and analyzed by ELISA with species-specific PCSK9 ELISA kit. Human PCSK9 was exclusively detectable in homozygous B-hPD-1/hPCSK9 mice and B-hPCSK9 mice but not in wild-type mice.

      Strain specific PD-1 expression analysis in homozygous B-hPD-1/hPCSK9 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPCSK9 mice (H/H;H/H) stimulated with Anti-CD3ε in vivo (7.5μg/mice, stimulation for 24h, i.p.), and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Human PD-1 was exclusively detected in T cells in homozygous B-hPD-1/hPCSK9 mice but not in wild-type mice. Mouse PD-1 was detected only in wild-type mice.

      Strain specific PD-1 expression analysis in homozygous B-hPD-1/hPCSK9 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPCSK9 mice (H/H;H/H) stimulated with Anti-CD3ε in vivo (7.5μg/mice, stimulation for 24h, i.p.), and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Human PD-1 was exclusively detected in CD4+ T cells in homozygous B-hPD-1/hPCSK9 mice but not in wild-type mice. Mouse PD-1 was detected only in wild-type mice.

      Strain specific PD-1 expression analysis in homozygous B-hPD-1/hPCSK9 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPCSK9 mice (H/H;H/H) stimulated with Anti-CD3ε in vivo (7.5μg/mice, stimulation for 24h, i.p.), and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Human PD-1 was exclusively detected in CD8+ T cells in homozygous B-hPD-1/hPCSK9 mice but not in wild-type mice. Mouse PD-1 was detected only in wild-type mice.

      Strain specific PD-1 expression analysis in homozygous B-hPD-1/hPCSK9 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPCSK9 mice (H/H;H/H) stimulated with Anti-CD3ε in vivo (7.5μg/mice, stimulation for 24h, i.p.), and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Human PD-1 was exclusively detected in Treg cells in homozygous B-hPD-1/hPCSK9 mice but not in wild-type mice. Mouse PD-1 was detected only in wild-type mice.